Nucleotide Excision Repair Is a Predominant Mechanism for Processing Nitrofurazone-Induced DNA Damage in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Nucleotide excision repair is a predominant mechanism for processing nitrofurazone-induced DNA damage in Escherichia coli.
Nitrofurazone is reduced by cellular nitroreductases to form N(2)-deoxyguanine (N(2)-dG) adducts that are associated with mutagenesis and lethality. Much attention recently has been given to the role that the highly conserved polymerase IV (Pol IV) family of polymerases plays in tolerating adducts induced by nitrofurazone and other N(2)-dG-generating agents, yet little is known about how nitrof...
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Localized DNA melting may provide a general strategy for recognition of the wide array of chemically and structurally diverse DNA lesions repaired by the nucleotide excision repair (NER) pathway. However, it is not clear what causes such DNA melting and how it is driven. Here, we show a DNA wrapping-melting model supported by results from dynamic monitoring of the key DNA-protein and protein-pr...
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Following low levels of UV exposure, Escherichia coli cells deficient in nucleotide excision repair (uvrA6 mutants) recover and synthesize DNA at near wild type levels, an observation that formed the basis of the post-replication recombination repair model. In this study, we characterized the DNA synthesis that occurs following UV-irradiation in the absence of nucleotide excision repair and sho...
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The entire process of nucleotide excision repair (NER) in Escherichia coli has been reconstituted in vitro from purified proteins and defined DNA substrates. However, how this system is organized in vivo in unclear. We report here the isolation and characterization of macromolecular assemblies containing NER and transcription proteins from E. coli. This ensemble consists of at least 17 proteins...
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Nucleotide excision repair (NER)l in all organisms, is able to accommodate to virtually any kind of DNA damage unlike many repair systems whose specificity is limited to a single species of damage. As a consequence NER has the advantage of being effective in responding to new types of environmental agents able to damage DNA. The model repair system under extensive study is the Escherichia coli ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2009
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.00495-09